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• #BETA ACTIN WESTERN BLOT HOW TO#

While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. Due to the cellular localization of β-Actin protein, the β-Actin Antibody (C4) shows cytoplasmic and membrane staining. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. The actin protein often constitutes as much as 50% of total cellular protein. β-Actin is highly conserved among species, making the β-Actin Antibody (C4) suitable for detection of β-Actin expressed in both mammalian and non-mammalian cells. β-Actin belongs to the actin protein family, which is expressed by all eukaryotic cells. β-Actin Antibody (C4) is available as both the non-conjugated anti-beta-Actin antibody form, as well as multiple conjugated forms of anti-beta-Actin antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor ® conjugates. Β-Actin Antibody (C4) is a high quality monoclonal beta-Actin antibody suitable for the detection of the beta-Actin protein of mouse, rat, human, avian, bovine, canine, porcine, rabbit, Dictyostelium discoideum and Physarum polycephalum origin. These reagents are now offered in bundles with β-Actin Antibody (C4) ( see ordering information below).

#BETA ACTIN WESTERN BLOT HOW TO#

Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of β-Actin (C4): sc-47778.

TransCruz reagent for Gel Supershift and ChIP applications (sc-47778 X, 200 µg/0.1 ml).also available conjugated to biotin for WB, IHC(P) and ELISA.also available conjugated to Alexa Fluor ® 680 or Alexa Fluor ® 790 for WB (NIR), IF and FCM for use with Near-Infrared (NIR) detection systems, such as LI-COR ®Odyssey ®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems.also available conjugated to Alexa Fluor ® 488, Alexa Fluor ® 546, Alexa Fluor ® 594 or Alexa Fluor ® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems.Anti-beta-Actin Antibody (C4) is available conjugated to agarose for IP HRP for WB, IHC(P) and ELISA and to either phycoerythrin or FITC for IF, IHC(P) and FCM.Anti-beta-Actin Antibody (C4) is recommended for detection of β-Actin of mouse, rat, human, avian, bovine, canine, porcine, rabbit, Dictyostelium discoideum and Physarum polycephalum origin by WB, IP, IF, IHC(P) and ELISA may cross-react with all six known isoforms of Actin in higher vertebrates (including cytoplasmic β- and γ- Actin isoforms, skeletal, cardiac, and vascular α-Actin isoforms, and enteric γ-Actin isoform).raised against gizzard Actin of chicken origin.Anti-β-Actin Antibody (C4) is a mouse monoclonal IgG 1 κ β-Actin antibody, cited in 12,639 publications, provided at 200 µg/ml.NITROCELLULOSE, PVDF AND NYLON MEMBRANES.AUTORADIOGRAPHY FILM, TAPE AND PLASTIC WRAP.Biologically Active Proteins & Peptides.NUCLEOTIDES, PEPTIDES, PROTEINS & AMINO ACIDS.BIOLOGICALLY ACTIVE & PURIFIED PROTEINS.PROTEIN A, G-PLUS & L AGAROSE IP REAGENTS.ISOTYPE SPECIFIC MOUSE IgG BINDING PROTEINS.POLYCLONAL GOAT/RABBIT ANTI-MOUSE SECONDARY ANTIBODIES.MONOCLONAL MOUSE ANTI-RABBIT/GOAT SECONDARY ANTIBODIES.